: Guidelines for preparing inoculum (such as matching a 0.5 McFarland standard) and isolation streaking methods for quality verification. Key Specifications
Selective media for pathogenic Neisseria (e.g., Modified Thayer-Martin) Certain specialized broths and transport media Steps for Implementing CLSI M22-A3 in Your Lab Step 1: Visual Inspection and Log-In
Non-Exempt media possess a higher inherent risk of failure, complex formulations, or specific nutritional requirements that degrade easily. Laboratories perform in-house QC on every new lot number of these media. Examples include: Campylobacter Agar clsi m22a3 pdf
Upon receipt, check the shipment for physical damage, frozen agar, excessive condensation, or cracked plates.
Exempt media consists of highly stable formulations with a proven track record of minimal failure rates. Laboratories do not need to perform full user QC (such as inoculation with control organisms) on these media if they receive documentation from the manufacturer proving the lot passed QC. Examples of commonly exempt media include: Sheep Blood Agar (SBA) MacConkey Agar (MAC) : Guidelines for preparing inoculum (such as matching a 0
Ensures that fastidious or critical pathogens are not missed due to substandard or degraded culture media.
What specific (e.g., CAP, CLIA) is auditing your laboratory? Examples include: Campylobacter Agar Upon receipt, check the
Before any organism is inoculated, the laboratory staff must check the media for:
CLSI M22-A3 is the third edition of the approved standard developed by the Clinical and Laboratory Standards Institute. It establishes guidelines for both manufacturers and end-user laboratories regarding the quality control responsibilities for commercially prepared microbiological culture media.
Maintain a certified Quality Management System (QMS), such as ISO 13485.
The CLSI M22-A3 guideline covers essential topics, including: